Figure 1. Elution profile of porcine enamel extracts from Sephadex G-100 column and ALP activity of ST2 cells induced by each fraction. (A) The chromatogram represents absorbance at 280 nm. Bars show ALP activities of ST2 cells exposed to G-100 fractions. Data are means ± SE of three culture wells. SDS-PAGE profiles of fractions of I to V (B) and G-100 fractions from No. 36 to No. 50 (C) stained with CBB. Molecular weights (Bio-Rad Low Range Standards, Hercules, CA, USA) are shown in left margin.
