Figure 3. Effect of HIP/L29 peptide on bFGF-induced proliferation. Gingival fibroblasts were plated in 24-well plates at an initial density of 3500 cells/well and allowed to attach as above. Subsequently, cells were rinsed twice with PBS and the medium was changed to 0.2% (v/v) FBS. HIP/L29 peptide (CRPKAKAKAKAKDQTK) (A) and BSA-conjugated peptide (AKAK-BSA) (B) were added to medium at the indicated concentrations in the presence of 50 ng/mL bFGF. On the indicated days, cells were trypsinized and counted. While peptide conjugated to BSA was very effective in reducing cell growth (bottom panel), unconjugated peptide had little effect (top panel). Data are presented as the means ± SD of 6 experiments. ***p < 0.0001 relative to corresponding day of group receiving bFGF only.
