Figure 4. Matrix metalloproteinase (MMP) secretion of F. necrophorum-treated epithelial cells. Confluent cultures of porcine periodontal ligament epithelial cells were exposed to F. necrophorum (AHN 12454) for 20 hrs. Untreated cells served as control. Medium aliquots of 25 µL were analyzed for matrix metalloproteinases, by means of gelatin zymography. Known molecular sizes of proMMP-9, -2, and –7 are indicated.

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