Figure 2. Immunoblotting of tropoelastin (upper panel). HGF and HPLF underwent lysis at the indicated times, and equal amounts of protein (100 µg) were separated by SDS-PAGE and transferred to PVDF membranes. The blots were probed with an anti-tropoelastin antibody and developed with ECL reagent. To ensure that equivalent amounts of extracted proteins were present in each lane, we stained another PVDF membrane with Amido Black 10B reagent before hybridization (lower panel). The results are representative of three independent experiments.
