Figure 1. Outline of and primers for real-time quantitative RT-PCR. The target for PCR was the 5' one-third region of the gtf genes shown at the top. Each gtf gene-specific primer (RT primer) corresponding to the 5' region of the catalytic domain (A) was used for the first-strand synthesis of cDNA (B). Real-time PCR was performed with the PCR primers corresponding to the hyper-variable region of each gtf gene (C). The expected sizes for each PCR product from gtfB, gtfC, and gtfD were 98 bp, 93 bp, and 83 bp, respectively (D).
