Figure 1. (A) Schematic drawing of ameloblast and odontoblast tissue preparations. The secretory ameloblast layer was dissected from the inner surface of the removed enamel organ epithelia (EOE), and the maturation ameloblast layer was prepared from the labial surface of the incisor. (B) The secretory ameloblast sample was prepared from the inner surface of the EOE, which contained secretory ameloblasts (SA), stratum intermedium (SI), and stellate reticulum cells (SR). After removal of all the cells covering the enamel matrix completely, the apical portion of the tooth was cut off, and the dental pulp was removed carefully. Almost all the odontoblasts remained on the surface of predentin matrix following the removal of the pulp tissue. (C) Cells remaining attached to the predentin matrix following removal of the dental pulp. Almost all the cells on predentin matrix are odontoblasts.
