Figure 3. The acidic, staurosporine-releasable Ca²⁺ store was the secretory granules in rat submandibular acinar cells. The rats were treated with isoproterenol (20 mg/kg, i.p.), and the acinar cells were obtained 2 hrs later by means of a method described in MATERIALS & METHODS. Fura-2-loaded cells were stimulated with 10 µM carbachol, and the perfusate Ca²⁺ was removed. Cells were then treated with 100 nM ionomycin, followed by 10 µM monensin (A) or 200 nM staurosporine (B) at the times indicated by the bars. The sizes of [Ca²⁺]_c increases induced by ionomycin+monensin and staurosporine observed in the degranulated cells were significantly smaller compared with those observed in control cells (Figs. 2C, 1A). Each trace is representative of five independent experiments.

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