Click on image to view larger version.
Figure 1. Fibrillin-1, fibrillin-2, and tropoelastin mRNA expression in human gingival fibroblasts (HGF) and human periodontal ligament fibroblasts (HPLF). (A) Northern blot analysis. HGF and HPLF were harvested at the time-points indicated. Total cell RNA was extracted, and 1 µg was analyzed by Northern blotting analysis as described in MATERIALS & METHODS. (B) Densitometric analysis of the time-course of changes in the levels of expression of fibrillin-1, fibrillin-2, and tropoelastin mRNA, measured as shown in (A). All mRNA expression levels were analyzed with the use of National Institutes of Health imaging software and were normalized relative to ß-actin mRNA expression. The levels of expression in the HGF two-week culture samples were arbitrarily assigned the value of 1. The results represent the means + SD (standard deviation) of three independent experimental determinations.
