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Figure 1. Schematic divisions of the ESEF and cell layer samples prepared from the labial side of a porcine incisor crown-stage tooth germ (a) and RT-PCR products of these samples, using the primers for EMSP1 (b) and enamelysin (c). 1, ESEF; 2, secretory ameloblast cell layer; 3, maturation-stage ameloblast cell layer; 4, odontoblast cell layer at the matrix formation stage; and 5, odontoblast cell layer at the maturation stage. Key for abbreviations: HME, highly mineralized enamel; EDJ, enamel-dentin junction; S, molecular standard.
