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Figure 2. Comparative cleavage rates of the 3 deca-peptides — FSM 
, GWL •, and WPA 
— by recombinant pig enamelysin (rpMMP-20). The rates, which are represented as the ratio of substrate at time t to substrate at time zero (St/S0) as a function of time, are exponential. The data presented in the curve are the means and standard deviations of from 3 to 8 determinations (except 5 hrs of FSM, 8 hrs of GWL, and 12 hrs of FSM, GWL, and WPA, each of which represents one determination). Statistical analysis (two-way ANOVA) revealed no significant difference among the rates of hydrolysis of the 3 peptide bonds by rpMMP-20. Cells had unequal sample sizes (n = 3-8). F = 2.27 (P > 0.10) for the differences among the peptides regardless of time, and F = 84.86 (P < 0.001) for the differences among times regardless of peptide.
