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Hemoglobin and LPS Act in Synergy to Amplify the Inflammatory Response

Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, Quebec, Canada G1K 7P4

View larger version (18K): [in this window] [in a new window]   Figure 1. Secretion of IL-1ß (A), TNF- (B), IL-6 (C), and IL-8 (D) by macrophages stimulated with LPS from P. gingivalis in the presence or absence of hemoglobin (10, 50, 100 µg/mL) for 24 hrs. Cytokine secretion was assessed by ELISA. The data are the means ± standard deviations of triplicate assays. *p < 0.05.

View larger version (18K): [in this window] [in a new window]   Figure 2. Secretion of IL-1ß (A), TNF- (B), IL-6 (C), and IL-8 (D) by macrophages stimulated with LPS from F. nucleatum in the presence or absence of hemoglobin (10, 50, 100 µg/mL) for 24 hrs. Cytokine secretion was assessed by ELISA. The data are the means ± standard deviations of triplicate assays. *p < 0.05.

View larger version (14K): [in this window] [in a new window]   Figure 3. Effect of hemoglobin on LPS-binding to macrophages. 3H-LPS from P. gingivalis and F. nucleatum were added to macrophages at a final concentration of 1 µg/mL in the presence or absence of hemoglobin (10, 50, 100 µg/mL). After 24 hrs, the quantity of 3H-LPS bound to macrophages was determined with the use of a multi-purpose scintillation counter. A value of 100% was assigned to the amount of LPS bound in the absence of hemoglobin. *p < 0.05.