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Accumulation of Topical Naproxen by Cultured Oral Epithelium

Section of Periodontology, College of Dentistry, The Ohio State University Health Sciences Center, 305 West 12th Avenue, P.O. Box 182357, Columbus, OH 43218-2357, USA

View larger version (9K): [in this window] [in a new window]   Figure 1. Time-course of naproxen accumulation by cultured SCC-25 monolayers. Cells were incubated for 10 min at 37°C in HBSS prior to the addition of 50 µg/mL naproxen. At the indicated times, the naproxen solution was removed, and extracellular naproxen was rapidly washed away. The data represent the mean of 6 experiments. Inset: Representative Lineweaver-Burk plot of naproxen transport by SCC-25 cells observed in a representative experiment conducted over a three-minute interval at 37°C.

View larger version (8K): [in this window] [in a new window]   Figure 2. Effect of pH on accumulation of naproxen by confluent SCC-25 monolayers. Cell monolayers were washed and overlaid with HBSS adjusted to the indicated pH. Kinetic analysis was performed as shown in Fig. 1, and the Vmax/Km ratio was used as an index of transport efficiency. The data are presented as the mean ± SEM of 5 experiments. Relative to pH 7.3, transport efficiency was significantly higher at pH 6.3 and 6.8 (P < 0.003, repeated-measures ANOVA), but similar at pH 7.8 and 8.3 (P > 0.3).

View larger version (18K): [in this window] [in a new window]   Figure 3. The effect of buffer modifiers on naproxen accumulation by SCC-25 cells. (Upper panel). The effects of varied (from 0 to 10%) concentrations of ethanol and glycerol on naproxen accumulation by SCC-25 cells. Naproxen accumulation was monitored over 3 min in the presence of the indicated additions to HBSS, under experimental conditions similar to those used in Fig. 1. (Lower panel) The effects of glucosylceramide and Pluronic F-127 (from 0 to 0.1%) on naproxen accumulation by SCC-25 cells. Treatment effects by all 4 modifiers were significant (P < 0.003, repeated-measures ANOVA). Except for 2% ethanol, every individual treatment was significantly different compared with controls (P < 0.05, Holm-Sidak test). The data are presented as the mean ± SEM of 6 experiments.

View larger version (13K): [in this window] [in a new window]   Figure 4. Comparison of the individual and combined effects of glycerol and Pluronic F-127 and the effects of glucocylceramide and acidic pH on naproxen accumulation by SCC-25 cells. The data represent the mean ± SEM of 5 experiments. Treatments significantly different from control are indicated by * (P < 0.05, Holm-Sidak post-test).