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Protective Effect of the Dental Pellicle against Erosive Challenges in situ

¹ Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil;
² Oral Health Research Institute, Indiana University School of Dentistry, 415 N. Lansing Street, Indianapolis, IN 46202-2876, USA; and
³ Faculty of Dentistry of Ribeirão Preto, State University of São Paulo, Ribeirão Preto, SP, Brazil

View larger version (10K): [in a new window]   Figure 1. Experimental design.

View larger version (52K): [in a new window]   Figure 2. Schematic view of the palatal appliance loaded with the holders.

View larger version (11K): [in a new window]   Figure 3. Data of enamel microhardness change. Values adjusted to quadratic fits (with pellicle—p < 0.05, r2 = 0.66; w/out pellicle—p < 0.05, r2 = 0.61) toward the erosive challenges. Average (SD) values of groups: (with pellicle) 0, –20.92 (7.15); 10, –34.68 (15.14); 20, –50.48 (16.44); 30, –70.94 (14.27); (w/out pellicle) 0, –19.90 (8.13); 10, –49.12 (15.66); 20, –60.51 (20.18); 30, –73.56 (19.06) (n = 12). Different letters at each erosive challenge mean statistical difference (p < 0.05).

View larger version (12K): [in a new window]   Figure 4. Dentin analysis. (top) Data of mineral loss (vol% mineral. µ m) in dentin. Values adjusted to linear fits (with pellicle—p < 0.05, r2 = 0.77; w/out pellicle—p < 0.05, r2 = 0.67) toward the erosive challenges. Average (SD) values of groups: (with pellicle) 0, 88.39 (18.00); 10, 142.00 (20.87); 20, 190.68 (42.33); 30, 226.95 (29.31); (w/out pellicle) 0, 98.62 (20.08); 10, 135.86 (27.03); 20, 185.40 (43.33); 30, 231.09 (46.53) (n = 12). (bottom) Data on lesion depth (µm) in dentin. Values adjusted to linear fits (with pellicle—p < 0.05, r2 = 0.78; w/out pellicle—p < 0.05, r2 = 0.71) toward the erosive challenges. Average (SD) values of groups: (with pellicle) 0, 5.95 (1.58); 10, 11.02 (1.86); 20, 14.80 (2.85); 30, 18.02 (3.07); (w/out pellicle) 0, 6.18 (1.33); 10, 11.29 (2.15); 20, 15.20 (3.84); 30, 18.55 (3.77) (n = 12).