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Human Saliva Proteome and Transcriptome

¹ School of Dentistry and Dental Research Institute,
² UCLA Mass Spectrometry and Proteomics Center,
³ Department of Chemistry and Biochemistry,
⁴ Department of Biological Chemistry, School of Medicine,
⁵ Jonsson Comprehensive Cancer Center,
⁶ Molecular Biology Institute;
⁷ Division of Head & Neck Surgery/Otolaryngology, School of Medicine, and
⁸ Henry Samueli School of Engineering and Applied Science, University of California Los Angeles, Los Angeles, CA 90095–1668, USA

View larger version (10K): [in a new window]   Figure 1. 2-D virtual display (pI vs. log Mr) of all identified proteins in human saliva (A) (n = 1), as well as the proteins complementary to the Normal Saliva Core Transcriptome list (B) (n = 10).

View larger version (54K): [in a new window]   Figure 2. RT-PCR validation of randomly selected mRNAs in Participant 1 (A), Participant 2 (B), and Participant 3 (C). (A) Lanes 1 and 20 are DNA ladders. Saliva RNAs were detected in lanes 2 (HSPD1), 4 (DEFA3), 6 (ITGB6), 8 (KLK1), 10 (RPS11), 12 (TF), and 18 (HNP-3). LOC340333 (lane 14) and ALB (lane 16) were not detectable. Total salivary gland RNA (Clontech, Palo Alto, CA, USA) was used as a positive control, shown in lanes 3 (HSPD1), 5 (DEFA3), 7 (ITGB6), 9 (KLK1), 11 (RPS11), 13 (TF), 15 (LOC340333), 17 (ALB), and 19 (Defensin, HNP-3), respectively. Negative controls were used in which input RNA was omitted, or in which RNA was used but reverse-transcriptase was omitted (data not shown). (B) Lanes 1 and 12 are DNA ladders. Lane 2 (TCN1), lane 4, (PIGR), lane 6 (MPO), lane 8 (HPX), and lane 10 (SERPINA1) are PCR products from total salivary gland RNA, which served as positive controls. Corresponding mRNAs of TCN1 (lane 3), MPO (lane 7), and HPX (lane 9) were detected in the participant’s saliva, but PIGR (lane 5) and SERPINA1 (lane 11) were not detected. (C) Lane 2 (CYST1), lane 4 (CYST2), lane 6 (CA4), lane 8 (HPX), and lane 10 (SERPINA1) are positive controls. CYST2 (lane 5), CA4 (lane 7), and HPX (lane 9) were detected in saliva, but CYST1 (lane 3) and SERPINA1 (lane 11) were not detectable.