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Immunogenicity and Persistence of a Targeted Anti-caries DNA Vaccine

Q.A. Xu^1,, F. Yu^2,, M.W. Fan^1,*, Z. Bian¹, Z. Chen¹, B. Fan¹, R. Jia¹, and J.H. Guo¹

¹ The Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Luoyu Road 237, 430079 Wuhan, Hubei, China; and
² now at Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;

View larger version (33K): [in a new window]   Figure 1. Antibody responses generated following intranasal delivery of bupivacaine:DNA complexes. BALB/c mice were immunized with 100 µg pGJA-P/VAX or pVAX1 mixed with bupivacaine on days 0 and 14. Serum and saliva samples were collected biweekly for ELISA. Values are expressed as the means plus standard deviations of the log10 antibody titer (5 animals per group). *Significantly different from control group (p < 0.01). days of immunization. (A) Serum IgG anti-PAc antibody levels. (B) Serum IgG anti-GTF-I antibody levels. (C) Salivary IgA anti-PAc antibody levels. (D) Salivary IgA anti-GTF-I antibody levels.

View larger version (11K): [in a new window]   Figure 2. PCR analysis of mouse nasal-associated lymphoid tissue and the draining cervical lymph node DNA samples extracted 6 mos after intranasal delivery of bupivacaine:DNA complexes. Lane 1, DNA marker DL2000; Lane 2, the nasal-associated lymphoid tissue DNA samples from pGJA-P/VAX-immunized mice; Lane 3, the nasal-associated lymphoid tissue DNA samples from normal mice spiked with pGJA-P/VAX; Lane 4, the nasal-associated lymphoid tissue DNA samples from pVAX1 vector-immunized mice; Lane 5, the draining cervical lymph node DNA samples from pGJA-P/VAX-immunized mice; Lane 6, the draining cervical lymph node DNA samples from normal mice spiked with pGJA-P/VAX; and Lane 7, the draining cervical lymph node DNA samples from pVAX1 vector-immunized mice.