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Gingival Tissue and Crevicular Fluid Co-operation in Adult Periodontitis

¹ Department of Medicine/Invärtes medicin, Helsinki University Hospital, Biomedicum Helsinki, PO Box 700 (Haartmaninkatu 8), FIN-00029 HUS, Helsinki, Finland;
² Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland;
³ Medico-social, Dental Clinic, Bogazici University, Istanbul, Turkey;
⁴ Department of Oral and Maxillofacial Diseases, HUCH, Institute of Dentistry, University of Helsinki, Box 41 FIN-00014 Helsinki, Finland;
⁵ University of Gazi, Faculty of Dentistry, Department of Periodontology, Ankara, Turkey;
⁶ TNO-Prevention and Health, Leiden, The Netherlands;
⁷ ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland; and
⁸ COXA Hospital for Joint Replacement, Tampere, Finland

View larger version (60K): [in a new window]   Figure 1. Western blot and zymography analysis of MMPs in gingival crevicular fluid from adult periodontitis patients and healthy controls. (A) Characterization of various molecular forms of MMP-3, -8, -9 in the GCF samples studied by Western blotting; and (B) characterization of MMP-9 studied by gelatin zymography (11% acrylamide separating gel containing 1 mg/mL gelatin). These experiments were done for seven periodontitis patients and five healthy controls.

View larger version (53K): [in a new window]   Figure 2. MMP-3 staining. (A) Intense immunoreactivity is seen in the adult periodontitis gingival tissue samples (n = 7). The fibroblast marked with an arrow is magnified 4x in the insert. (B) MMP-3 staining is weak in healthy tissue (n = 5), whereas (C) no staining is detected in the negative staining control. Scale bar = 100 µm.

View larger version (16K): [in a new window]   Figure 3. MMP-3, -8, and -9 activity (mean ± SEM) (A) without APMA and (B) with APMA in TNF--stimulated (+) gingival fibroblasts supernatant at 48 hrs compared with non-stimulated (–) cells. ***p < 0.001, **p < 0.01, *p < 0.05. NS = non significant.

View larger version (17K): [in a new window]   Figure 4. Schematic presentation of a mutual MMP activation cascade in periodontitis.