Polyelectrolyte Multilayer Film Coating and Stability at the Surfaces of Oral Prosthesis Base Polymers: an in vitro and in vivo Study
O. Etienne1,2,
C. Picart1,
C. Taddei2,
P. Keller1,
E. Hubsch3,
P. Schaaf3,
J.C. Voegel1,
Y. Haikel2,
J.A. Ogier1, and
C. Egles1,*
1 Institut National de la Santé et de la Recherche Médicale, Unité 595, 11, rue Humann, 67085 Strasbourg Cedex, France;
2 Faculté de Chirurgie Dentaire, Université Louis Pasteur, 1, Place de l’hôpital, 67000 Strasbourg, France; and
3 Institut Charles Sadron, UPR 22 CNRS, 6 rue Boussingault, 67083 Strasbourg Cedex, France
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Figure 2. Film presence on polymer surfaces. (A–E) Topography is observed by ESEM images (scale bars, 50 µm [x400]; inlays, 10 µm [x1600]). Below each main picture is a representation of the corresponding image of a CSLM vertical section of the polyelectrolyte film. G, comparative measurements of static contact angles. The smaller the contact angles, the greater the wettability.
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Figure 4. In vivo experiments in rats. PMMA discs were coated with Pre-(PGA-PLL)20-PGA-PLLFITC film, by an automated dipping process, to ensure a full coating (A) and were sutured to the rat’s cheek (B). The CSLM image (115 x 115 µm) of the disc surfaces after 4 days in the rat’s mouth, showing the film still intact and entirely covering the disc on the mucosal side (C), while only fragments are visible on the lingual side of the specimen (D). Scale bars: 10 µm.
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f at 15 Hz, 
= 3). The exponential regression follows the function: y = y0 + Aexp(Bx) (with y0 = –398.7, A = 431.8, B = 0.1, R = 0.999). (C) Vertical section obtained by confocal laser scanning microscopy (CLSM) observation through a Pre-(PGA-PLL)20-PGA-PLLFITC film on a glass substrate. The film thickness is about 1 µm.