HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Appendix Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Nakajima, T. Articles by Yamazaki, K. Search for Related Content PubMed PubMed Citation Articles by Nakajima, T. Articles by Yamazaki, K.

Regulatory T-cells Infiltrate Periodontal Disease Tissues

¹ Periodontology and Immunology, Department of Oral Health and Welfare, Niigata University Faculty of Dentistry, 5274 Gakkocho 2-ban-cho, Niigata 951-8514, Japan;
² General Dentistry and Clinical Education Unit, Niigata University Medical and Dental Hospital, Niigata, Japan;
³ Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan; and
⁴ Oral Biology and Pathology, School of Dentistry, The University of Queensland, Brisbane, Australia;

View larger version (101K): [in a new window]   Figure 1. Identification of Tr-cell-related markers in periodontitis lesions. Immunohistochemical double-staining for CD25 (blue)/CD4 (brown) (A), CTLA-4 (blue)/CD4 (brown) (B), and CTLA-4 (blue)/CD25 (brown) (C), and subsequent immunofluorescent staining by FITC-conjugated CD4 on the same section (D). Single-positive cells and double-positive cells are indicated by open arrowhead and closed arrowhead, respectively (A,B). Photographs of the same areas (C,D) showed CD4+CD25+CTLA-4+ triple-positive cells (closed arrowhead) and CD4+CD25+CTLA-4– double-positive cells (open arrowhead) in periodontitis lesions.

View larger version (28K): [in a new window]   Figure 2. Relationship between percentages of CD4+CD25+ cells (A,B) or CD4+CTLA-4+ cells (C,D) and B/T ratio, and CD4+CD25+ and CD4+CTLA-4+ cells (E,F). Fifty areas of 17 specimens of periodontitis and 15 areas of 9 specimens of gingivitis were analyzed. Each circle represents one area. Significantly positive correlation was observed between the percentages of double-positive cells and the B/T ratio in periodontitis (A,C). Significantly positive correlation was observed between the percentages of CD4+CD25+ cells and CD4+CTLA-4+ cells in both periodontitis and gingivitis (E,F). A,C,E: Periodontitis. B,D,F: Gingivitis.

View larger version (11K): [in a new window]   Figure 3. Gene expressions in periodontitis (N = 20) and gingivitis lesions (N = 21). The relative quantities of FOXP3 (A), TGF-ß1 (B), and IL-10 (C) were normalized to the relative quantity of GAPDH. Data are expressed as mean ± SE. The expressions of FOXP3 (p = 0.0325) and TGF-ß1 (p = 0.0475) were significantly higher in periodontitis than in gingivitis.