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PTH(1-34) Affects Osteoprotegerin Production in Human PDL Cells in vitro

Department of Orthodontics, Dental Clinic, University of Bonn, Welschnonnenstr. 17, 53111 Bonn, Germany;

View larger version (34K): [in a new window]   Figure 1. Effect of PTH(1-34) treatment on regulation of cell number in confluent and pre-confluent PDL cells. Cells were either treated intermittently with 10–12 M PTH(1-34) for 0, 1, 3, 6, or 24 hrs during 3 cycles of 48 hrs each, or were exposed continuously (cont). Data were acquired from one of two separate experiments, both yielding comparable results. Each value is the mean ± SEM for 6 independent cultures. *P < 0.05, experimental group vs. untreated control. #P < 0.05, experimental group vs. PTH(1-34) for 1 hr/cycle; •P < 0.05, experimental group vs. PTH(1-34) for 3 hrs/cycle.

View larger version (32K): [in a new window]   Figure 2. Decrease in osteocalcin production in confluent PDL cells following intermittent and continuous exposure to PTH(1-34) as opposed to a significant increase in response to 1 hr/cycle challenge in pre-confluent cells. Cells were either treated intermittently with 10–12 M PTH(1-34) for 0, 1, 3, 6, or 24 hrs during 3 cycles of 48 hrs each, or were challenged continuously (cont). Data were acquired from one of two separate experiments, both yielding comparable results. Each value is the mean ± SEM for 6 independent cultures. *P < 0.05, experimental group vs. untreated control; #P < 0.05, experimental group vs. PTH(1-34) for 1 hr, 3 hrs, 6 hrs/cycle.

View larger version (43K): [in a new window]   Figure 3. PTH (1-34) effect on OPG expression and production. (A) Serving as an example, the RT-PCR for OPG, RANKL, and GAPDH following PTH exposure of confluent PDL cells from one out of six donors is depicted. (B) Biphasic reduction of OPG mRNA expression following intermittent PTH(1-34) challenge of confluent PDL cells, as compared with no effect in pre-confluent PDL cells. PDL cells were treated intermittently with 10–12 M PTH(1-34) for 0, 1, 3, 6, or 24 hrs during 3 cycles of 48 hrs each, or were exposed continuously (cont). Data were taken from one of two separate experiments, both yielding comparable results. Each value is the mean ± SEM for 6 independent cultures. *P < 0.05, experimental group vs. untreated control; #P < 0.05, experimental group vs. PTH(1-34) for 1 hr/cycle; •P < 0.05, experimental group vs. PTH(1-34) for 6 hrs/cycle. (C) Time-dependent reduction in OPG protein levels in response to intermittent PTH(1-34) challenge of confluent PDL cells. In contrast, there was an increase in OPG protein production following intermittent PTH(1-34) exposure for 6 hrs/cycle, and also after continuous treatment in pre-confluent cells. PDL cells were treated intermittently with 10–12 M PTH(1-34) for 0, 1, 3, 6, or 24 hrs during 3 cycles of 48 hrs each, or were exposed continuously (cont). *P < 0.05, experimental group vs. untreated control; #P < 0.05, experimental group vs. intermittent PTH(1-34) exposure.