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Novel Bacterial Phylotypes in Endodontic Infections

¹ Department of Endodontics, Estácio de Sá University, Rio de Janeiro, Brazil; and
² Institute of Microbiobiology Prof. Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil;

View larger version (92K): [in a new window]   Figure 1. DGGE band profiles of amplified 16S rDNA from samples taken from infected root canals. Numbered bands were excised and sequenced.

View larger version (40K): [in a new window]   Figure 2. Neighbor-joining tree showing phylogeny of 16S rDNA sequences obtained directly from cases of primary endodontic infection. Sequences were aligned with ClustalW, and distances were calculated with the Jukes-Cantor algorithm. Five hundred bootstrap trees were generated, and bootstrap confidence levels as percentages (only values over 50%) are shown at tree nodes. Accession numbers for each 16S rDNA sequence are given. Scale bar shows number of nucleotide substitutions per site.