HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Appendix Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Nakamura, H. Articles by Ogawa, T. Search for Related Content PubMed PubMed Citation Articles by Nakamura, H. Articles by Ogawa, T.

Molecular and Biomechanical Characterization of Mineralized Tissue by Dental Pulp Cells on Titanium

The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, 10833 Le Conte Avenue (B3-081 CHS), Box 951668, Los Angeles, CA 90095-1668, USA;

View larger version (68K): [in a new window]   Figure 1. Surface morphology images of titanium disks used for cell culture. Scanning electron microscopic (SEM) images of the (A) polystyrene, (B) machined titanium surface, and (C) dual-acid-etched (DAE) titanium surface. Bar = 5 µm. Atomic force microscopic (AFM) images of the (D) polystyrene, (E) machined titanium, and (F) the DAE titanium. All AFM images were scanned in a 5 µm x 5 µm area, and images were constructed in a custom vertical scale; 15.64 nm for panel D, 146.49 nm for panel E, and 816.50 nm for panel F.

View larger version (39K): [in a new window]   Figure 2. Characterization of dental pulp cells. (A) Immunofluorescence flow-cytometric analysis of the dental pulp cells at day 1 culture on the polystyrene without dexamethasone and day 14 culture with or without dexamethasone. Immunoreaction was performed with CD44-specific monoclonal antibody conjugated with FITC (y axis). The vertical line was set to the reactivity level of < 1% mean fluorescence, obtained from the cell suspension without CD44 antibody. (B) Alkaline phosphatase activity of the DPCs. The left panels show representative images of ALP staining of the cells cultured on the polystyrene. The percentage of the ALP-positive area relative to the culture area was measured by means of a digital image analyzer. Data are shown as the mean ± SD (n = 3). (C) The left panels show representative images of Von Kossa staining of the cells cultured on the polystyrene. The percentage of the Von Kossa-positive area relative to the culture area was measured by means of a digital image analyzer. Data are shown as the mean ± SD (n = 3).

View larger version (93K): [in a new window]   Figure 3. Morphology and biomechanical properties of mineralized tissue by dental pulp cells on titanium. Scanning electron microscopic (SEM) images of days 14 (A,B,C), 28 (D,E,F), and 42 (G,H,I) mineralized tissue by dental pulp cells in 3 culture conditions. Tissue on the polystyrene (A,D,G) depicts accretion of fibrous and small globular (arrow) structures, and rounded cells (asterisk). Tissues on titanium (E,F,H,I) depict laminar structure with small globules (arrow) and rounded cells (asterisk) on the surface. The crack appearance is seen as an artifact due to tissue contraction. Fibrous structures embedded within the lamina (arrow) are seen in the tissue on titanium (I). Bar = 20 µm for all panels. (J) A representative elemental spectrum obtained from energy-dispersive spectroscopy (EDS) of day 42 mineralized tissue on the machined titanium. Ca: calcium. P: phosphorus. Ti: titanium. (K) Calcium-to-phosphorus molar ratio of mineralized tissue in the 3 different culture conditions. Data are shown as the mean ± SD (n = 3). (L,M) Biomechanical properties of the tissue cultured in 3 different culture conditions: polystyrene, machined titanium (machined Ti), or dual-acid-etched titanium (DAE Ti). Hardness (L) and elastic modulus (M) measured by 200 mN maximum load nano-indentation. Data are shown as the mean ± SD (n = 3).

View larger version (31K): [in a new window]   Figure 4. Expression of bone and dentin extracellular matrix (ECM)-related genes analyzed by reverse-transcriptase polymerase chain-reaction (RT-PCR). (A) A representative electrophoresis image visualized with ethidium bromide staining from triplicate PCR trials. (B) The expression time-course for each gene. The intensity of bands was normalized relative to the GAPDH expression level, and further normalized with respect to the lowest expression level of all detected bands.