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Tannerella forsythia-induced Alveolar Bone Loss in Mice Involves Leucine-rich-repeat BspA Protein

¹ Department of Oral Biology, School of Dental Medicine, State University of New York, 3435 Main Street, Buffalo, NY 14214, USA;
² Department of Microbiology, Oral Health Science Center, Tokyo Dental College, Chiba 261-8502, Japan; and
³ Department of Biology, Bates College, Lewiston, ME 04240, USA;

View larger version (29K): [in a new window]   Figure 1. Serum antibody levels in mice following infection/immunization. (A) Anti-whole-specific IgG, (B) rBspA-specific IgG, and (C) rBspA-specific serum IgA. Group A, control (sham-infected and sham-immunized); group B, T. forsythia 43037-infected; group C, BFM571-infected; group D, BspA-immunized and T. forsythia 43037-infected; group E, BspA-immunized. Bars represent mean ± standard error (n = 12).

View larger version (15K): [in a new window]   Figure 2. Detection of serum IgA by Western immunoblotting. The individual nitrocellulose strips electroblotted with rBspA protein following SDS-PAGE were probed with respective pooled mouse sera (1:100 dilution), followed by goat anti-IgA and color developed with TMB membrane reagent. 1, Group A, control; 2, Group B, T. forsythia- infected; 3, Group C, BFM-571-infected; 4, Group D, rBspA-immunized and T. forsythia-infected; and 5, Group E, rBspA-immunized.

View larger version (13K): [in a new window]   Figure 3. Proliferative responses of splenic lymphoid cells derived from mice (12 per group) to rBspA (A) and to ConA (B). Cultures were performed in quadruplicate, and the results are expressed as means of the stimulation index, determined as described in the text. Group A, control (sham-infected and sham-immunized); group B, T. forsythia-43037 infected; group C, BFM571-infected; group D, rBspA-immunized and T. forsythia 43037-infected; and group E, rBspA-immunized. **P < 0.005 and *P < 0.05, values significantly higher compared with group A.

View larger version (19K): [in a new window]   Figure 4. (A,B) Bone loss after infection with T. forsythia in BALB/cByJ mice. Sites 1, 2, & 3 are on first molars, sites 4 & 5 are on second molars, and sites 6 & 7 are on third molars. L, left; R, right. Data points represent the mean ± SEM from 8 mice. (A) Comparison between the T. forsythia 43037 (Tf43037)-infected and sham-infected mice. The CEJ:ABC was greater in Tf43037-infected mice than in sham-infected mice at every site, indicating bone loss. (B) Comparison between the mutant BFM571- and sham-infected mice. (C) Comparison of total horizontal bone loss between groups calculated as the average of 14-site total CEJ-ABC distance for each group. Data represented as the millimeter bone loss per site per group. Significant T. forsythia 43037-induced alveolar bone loss was observed as compared with that in sham-infected controls. Bone loss in mice infected with the mutant strain BFM571 was not significantly different from that in sham-infected mice. Immunization with rBspA protein significantly reduced T. forsythia 43037-induced bone loss in mice. *Values significantly greater than in sham-infected controls (P < 0.05). P = NS, not significant compared with controls.