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Penetration of Fluoride into Natural Plaque Biofilms

P.S. Watson1,*, H.A. Pontefract2, D.A. Devine1, R.C. Shore1, B.R. Nattress2, J. Kirkham1, and C. Robinson1

1 Division of Oral Biology and 2 Department of Restorative Dentistry, Leeds Dental Institute, Clarendon Way, Leeds, LS29LU, UK;



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Figure 1. Biomass distribution in plaque biofilms. (a) Mean biomass volume throughout the depth of plaque biofilms (n = 48, error bars show ± SEM). (b) Toluidine-blue-stained sections from a typical plaque biofilm. Sections i–vi taken at distances (from enamel surface) of approximately 820 µm, 730 µm, 560 µm, 390 µm, 220 µm, and 110 µm, respectively.

 


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Figure 2. Mean fluoride concentration profiles throughout the depth of plaque biofilms (n = 6, error bars show ± SEM). (a) Biofilms exposed to NaF for 30 sec, ± wash in saliva-like solution. (b) Biofilms exposed to NaF for 120 sec, ± wash in saliva-like solution. (c) Unexposed controls, and biofilms exposed to NaF for 30 min.

 


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Figure 3. Mean biomass-associated fluoride concentration profiles throughout the depth of plaque biofilms (n = 6, error bars show ± SEM). (a) Biofilms exposed to NaF for 30 sec, ± wash in saliva-like solution. (b) Biofilms exposed to NaF for 120 sec, ± wash in saliva-like solution. (c) Unexposed controls, and biofilms exposed to NaF for 30 min.

 





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