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Continuing Etching of an All-in-One Adhesive in Wet Dentin Tubules

¹ Department of Oral Biology and
² Department of Pediatric Dentistry, University of Missouri-Kansas City School of Dentistry, 650 E. 25th St., Kansas City, MO 64108, USA;

View larger version (133K): [in a new window]   Figure 1. Light micrographs of Prompt L-Pop/dentin interfaces stained with Goldner’s trichrome. (A,A') Sectioned after 24 hrs in normal saline: mineralized dentin (green), adhesive (pale beige), interface (pale red); arrow indicates adhesive penetration into tubule. (B,B') Sectioned after 4 wks in normal saline. The last step in the process, which is the light green component, was not used. It showed very poor structural integrity following 4 wks’ aqueous storage.

View larger version (152K): [in a new window]   Figure 2. Scanning electron micrographs of Prompt L-Pop/dentin interfaces of cross-sections from the same tooth with different aqueous storage times. (A) The interface was treated with 5 N HCl and 5% NaOCl after 24 hrs in normal saline. (B) The interface was treated only with 5% NaOCl after 24 hrs in normal saline. (C,D) The interface was treated only with 5% NaOCl after 4 wks in normal saline.

View larger version (27K): [in a new window]   Figure 3. In situ micro-Raman spectra of Prompt L-Pop adhesive recorded at the spot close to the adhesive/dentin interface and ~ 20 and 50 µm deep to the surface within the tubules.

View larger version (27K): [in a new window]   Figure 4. Effect of water on degree of conversion of Prompt L-Pop adhesive following 20-second light curing. (A) The Raman spectra of the mixtures of Prompt L-Pop adhesive with different water contents (0–60 vol%). The spectrum of unpolymerized adhesive is also presented. (B) Degree of conversion as a function of water content in Prompt L-Pop. It was noted that the DC of Prompt L-Pop adhesive is not the highest in the absence of water. This may be associated with the effect of viscosity on transportation of monomers to propagating chains. Values are means ± standard deviation (n = 6 for each cell of data).