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Painful Tooth Stimulation Elevates Matrix Metalloproteinase-8 Levels Locally in Human Gingival Crevicular Fluid

¹ Institute of Dentistry, PO Box 41, 00014 University of Helsinki, Finland;
² Department of Oral and Maxillofacial Diseases, Helsinki University Central Hospital (HUCH), Helsinki, Finland;
³ Department of Physiology and Experimental Pathophysiology, University of Erlangen/Nuernberg, Germany; and
⁴ Finnish Student Health Service;

View larger version (22K): [in a new window]   Figure 1. Matrix metalloproteinase (MMP)-8 level in gingival crevicular fluid (GCF) and its spatial relation to painful tooth stimulation. Average changes (mean ± SEM, n = 8) of MMP-8 levels in GCF of different teeth during painful stimulation of tooth 11. Shown are the relative changes from baseline during stimulation, 4 min, and 8 min after the end of stimulation. The stars mark significant differences as compared with baseline (Wilcoxon matched-pairs). Data for the Fig. were counted from quantitative IFMA values normalized to baseline (baseline = 100%).

View larger version (59K): [in a new window]   Figure 2. A representative example of changes in Western immunoblots for molecular forms of MMP-8 from the stimulated tooth. The GCF samples contained bands at 60–80 kDa, corresponding to PMN-type active and pro-enzymes, and at 45–55 kDa, corresponding to mesenchymal-type (non-PMN) active and pro-enzymes. PMN-type isoform was slightly elevated during tooth stimulation (lane 5), and non-PMN-type MMP-8 was elevated after the end of tooth stimulation (lanes 6, 7). PMN indicates PMN-type MMP-8 (lane 1), and F indicates mesenchymal-type MMP-8 (lane 8) from cultured human rheumatoid synovial fibroblasts. Lanes 2 to 7 represent molecular forms of MMP-8 in baseline prior to (pre; lanes 2 and 3), during (stim; lanes 4 and 5), 4 min after (post; lane 6), and 8 min after (post, lane 7) tooth stimulation. Mobilities of molecular-weight markers appear at the left.