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Expression of Protein Kinases C ßI, ßII, and VEGF during the Differentiation of Enamel Epithelium in Tooth Development

Department of Oral Pathology, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan;

View larger version (169K): [in a new window]   Figure 1. Immunohistochemical analysis of PKC ßI (a,b,c) and ßII (d,e,f) expression in the rat tooth germ at the cap (a,d), early bell (b,e), and late bell (c,f) stages (scale bars, 100 µm). At the cap stage, no immunoreactivity for PKC ßI was seen (a). At the early bell stage, the inner enamel epithelium in the cervical loop shows strong immunoreactivity for PKC ßI (b'). At the late bell stage, the inner enamel epithelium in the cervical loop in the dental papilla shows weak immunoreactivity for PKC ßI (c'). At the cap stage, no immunoreactivity for PKC ßII can be seen (d). At the early bell stage, the inner enamel epithelium in the cervical loop shows strong immunoreactivity for PKC ßII (e'). At the late bell stage, the inner enamel epithelium in the cervical loop shows weak immunoreactivity for PKC ßII (f').

View larger version (86K): [in a new window]   Figure 2. Cells of the tooth germ at the cap stage before (a) and after (b) laser microdissection (scale bars, 100 µm). The white areas in Fig. 2b are areas that had been microdissected. Comparison of the levels of mRNA expression of PKC ßI, PKC ßII, amelogenin (c), and VEGF (d) in the outer enamel epithelium, stellate reticulum, inner enamel epithelium, and dental papilla, with the use of real-time RT-PCR. Data are the mean ± SEM from 5 experiments. PKC ßI and PKC ßII expression levels are not appreciably up-regulated in any of the four areas.

View larger version (94K): [in a new window]   Figure 3. Cells of the tooth germ at the early bell stage before (a) and after (b) laser microdissection (scale bars, 100 µm). The white areas in Fig. 3b are areas that had been microdissected. Comparison of the levels of mRNA expression of PKC ßI, PKC ßII, amelogenin (c), and VEGF (d), with the use of real-time RT-PCR analysis. Data are the mean ± SEM of 5 experiments. PKC ßI, PKC ßII, VEGF, and amelogenin expression are appreciably up-regulated in the inner enamel epithelium (P < 0.05).

View larger version (82K): [in a new window]   Figure 4. Cells of the tooth germ at the late bell stage before (a) and after (b) laser microdissection (scale bars, 500 µm). The white areas in Fig. 4b are areas that had been microdissected. Comparison of the levels of mRNA expression of PKC ßI, PKC ßII, amelogenin (c), and VEGF (d), with the use of real-time RT-PCR analysis. Data are the mean ± SEM of 5 experiments. The expression levels of PKC ßI, PKC ßII, VEGF, and amelogenin were reduced in the inner enamel epithelium from the early bell stage; however, they remained higher than in other areas of the dental germ.