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Role of TNF- and Its Receptors in Pericoronitis

¹ Department of Medicine/Invärtes medicin, Helsinki University Hospital, Helsinki, Finland;
² Institute of Biomedicine/Anatomy, University of Helsinki, Helsinki, Finland;
³ Medico-social Centre, Dental Clinic, Bogazici University, Istanbul, Turkey;
⁴ Finnish Student Health Service, Helsinki, Finland;
⁵ ORTON Orthopaedic Hospital of the Invalid Foundation, Helsinki, Finland; and
⁶ COXA Hospital for Joint Replacement, Tampere, Finland

View larger version (159K): [in a new window]   Figure 1. Tumor necrosis factor- (TNF-) and its receptors in pericoronitis. (A) TNF- is localized in basal and suprabasal cells, basement membrane, monocyte/macrophage-, fibroblast-like, and vascular endothelial cells. Upper and lower inserts: Twice-magnified images of TNF--positive cells in the lamina propria. (B) No staining was detected in the negative control. (C) TNF-R1 in serial tissue sections is localized in monocyte/macrophage- and fibroblast-like cells, and vascular endothelial and basal epithelial cells. Upper and lower inserts: Twice-magnified images of TNF-R1-positive cells in the lamina propria. (D) TNF-R2 in a serial section is localized in monocyte/macrophage-and fibroblast-like cells, and vascular endothelial and basal epithelial cells. Upper and lower inserts: Twice-magnified images of TNF-R2-positive cells in the lamina propria. Scale bar = 200 µm.

View larger version (161K): [in a new window]   Figure 2. TNF- and its receptors in healthy control samples. (A) TNF- is almost exclusively localized in fibroblast-like cells and vascular endothelial cells. (B) TNF-R1 in a serial section is localized in stromal fibroblast-like cells and vascular endothelial cells. (C,D) Larger magnifications from the same samples but from different areas, showing TNF- and TNF-R1 staining, respectively. (E) In a serial section, weak TNF-R2 staining was occasionally found in a few stromal fibroblast- and macrophage-like, vascular endothelial, and basal epithelial cells. Scale bar = 200 µm.

View larger version (146K): [in a new window]   Figure 3. IL-1ß and VCAM-1 in pericoronitis (A,B, respectively) and healthy tissues (C,D, respectively). In pericoronitis (A), IL-1ß was found in fibroblast - and macrophage-like, vascular endothelial, and epithelial cells. (B) VCAM-1 was mainly localized in endothelial cells of the subepithelial blood vessels. In healthy controls (C), most of the IL-1ß-positive cells were vascular endothelial cells. Very few and weakly IL-1ß-positive subepithelial cells were observed in healthy controls. (D) VCAM-1 was rare. Scale bar = 200 µm; inserts were twice-magnified.

View larger version (43K): [in a new window]   Figure 4. Gelatinolytic activities of the cultured tissue supernatants were studied with zymography. (A) Representative results from non-stimulated (–) and TNF--stimulated (+) healthy tissue sample supernatants at 48 hrs. (B) Inflamed tissue without (–) and with (+) TNF- blocker showed reverse findings at 48 hrs. Molecular-weight standards (kDa) are marked to the left (7.5% acrylamide separating gel containing 1 mg/mL gelatin).