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Heterogeneity of IgG Glycosylation in Adult Periodontal Disease

¹ Departments of Microbiology-Box 1, ² Medicine, and ³ Oral Diagnostics, University of Alabama at Birmingham, 845 19th Street South, Birmingham, AL 35294-2170;

View larger version (13K): [in a new window]   Figure 1. Structure of N-linked glycans of IgG and analyses of IgG galactose deficiency. (A) Example of complex type N-linked glycans. Abbreviations: N-acetylglucosamine (GlcNAc), galactose (Gal), sialic acid (SA), mannose (Man), fucose (Fuc), asparagine (Asn). Variably present saccharides are in parentheses. Fc region-associated glycans of IgG are usually not sialylated, and only some of them are fully galactosylated or with bisecting N-acetylglucosamine. Galactose-deficient IgG glycans are terminated by N-acetylglucosamine residues that react with PVL lectin, while fully galactosylated glycans do not react with PVL. (B) Correlation between PVL reactivity (OD 405 nm, 30 min) and gas-liquid monosaccharide composition (mannose/galactose ratio) of differently galactosylated purified IgGs. Pierson’s correlation coefficient, r = 0.73 (N = 18).

View larger version (10K): [in a new window]   Figure 2. Galactose deficiency of IgG in serum and gingival crevicular fluid of periodontal disease patients and controls. (A) PVL reactivity (OD at 405 nm after 2 hrs of incubation) of serum IgG from patients with periodontal disease (N = 14) and from healthy controls (N = 10). P = 0.0046. (B) IgG glycosylation (PVL index) in serum and gingival crevicular fluid (GCF) from periodontal patients (N = 7).

View larger version (69K): [in a new window]   Figure 3. Paired immunofluorescence staining for IgG with FITC-conjugated (green) F(ab')2 fragment of anti-human IgG and galactose-deficient N-linked glycans disclosed by biotinylated PVL and AMCA-labeled avidin (blue) of gingivae from patients with advanced periodontal disease. The picture shows a representative example of inflamed periodontal disease tissue (N = 7). Most of the IgG-containing cells (green) co-stain for galactose-deficiency (blue). Arrows point to IgG-containing cells that do not stain with PVL. Bar size, 8 µm.