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Effects of Cyclosporin-A-induced Immunosuppression on Periapical Lesions in Rats

¹ Departments of Restorative Dentistry and Endodontology, and
² Oral and Maxillofacial Radiology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan;

View larger version (27K): [in a new window]   Figure 1. Lesion volume measurements. (a) CT images analyzed by the image analysis system. (b) Data are expressed as the percentages of lesion volume at week 0 (weeks 0–4, n = 10; weeks 4–8, n = 4) and shown as means ± SD. Statistical analyses were performed by means of the Dunnett test. Significance (*) was determined at a level of p < 0.05.

View larger version (103K): [in a new window]   Figure 2. Pathological evaluation. (a,b) Control group at week 4. (c,d) 20 mg/kg/day group at week 4. (e,f) Control group at week 8. (g,h) 20 mg/kg/day group at week 8. Panels b, d, f, and h are high-magnification views of the periapical lesions shown in panels a, c, e, and g, respectively. AA, Apical Abscess; CE, Cementum; B, Bone. Scale bars: a, c, e, g = 250 µm; b, d, f, h = 50 µm.

View larger version (90K): [in a new window]   Figure 3. Immunohistochemical staining at week 4. (a) Anti-CD3 staining of the control group. (b) Anti-CD3 staining of the 20 mg/kg/day group. (c) Anti-CD25 staining of the control group. (d) Anti-CD25 staining of the 20 mg/kg/day group. Scale bars = 100 µm. (e) CD3+ cell-counts in the lesions of each group (n = 6). (f) CD25+ cell-counts in the lesions of each group (n = 6). (g) ED1+ cell-counts in the lesions of each group (n = 6). (h) TRAP staining of the control group. (i) TRAP staining of the 20 mg/kg/day group. Values are the means ± SD. *A value of p < 0.01 was determined as statistically different in comparison with the controls.

View larger version (100K): [in a new window]   Figure 4. Immunohistochemical staining at week 8. (a) Anti-CD3 staining of the control group. (b) Anti-CD3 staining of the 20 mg/kg/day group. (c) Anti-CD25 staining of the control group. (d) Anti-CD25 staining of the 20 mg/kg/day group. Scale bars = 100 µm. (e) CD3+ cell-counts in the lesions of each group (n = 4). (f) CD25+ cell-counts in the lesions of each group (n = 4). (g) ED1+ cell-counts in the lesions of each group (n = 4). (h) TRAP staining of the control group. (i) TRAP staining of the 20 mg/kg/day group. Values are the means ± SD. *p < 0.05. **A value of p < 0.01 was determined as statistically different in comparison with controls.