HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Full Text (PDF) Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Mahanonda, R. Articles by Pichyangkul, S. Search for Related Content PubMed PubMed Citation Articles by Mahanonda, R. Articles by Pichyangkul, S.

Monocyte Activation by Porphyromonas gingivalis LPS in Aggressive Periodontitis with the Use of Whole-blood Cultures

¹ Department of Periodontology, Faculty of Dentistry, Chulalongkorn University, Henry Dunant Rd., Bangkok 10330, Thailand;
² Immunology Lab, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand;
³ Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Bangkok, Thailand;
⁴ Department of Periodontics & Endodontics, State University of New York at Buffalo, Buffalo, NY, USA;
⁵ Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand;

View larger version (22K): [in a new window]   Figure 1. Whole-blood vs. PBMC cultures after incubation with P. gingivalis LPS (10 ng/mL) for 48 hrs. Monocytes were analyzed for CD40 and CD80 expression by flow cytometry. (A) Representative histograms comparing whole-blood with PBMC cultures (n = 3). Dotted lines are isotype controls; the shaded areas are media controls; the solid lines represent cultures incubated with P. gingivalis LPS. (B) The mean MFI-fold increase of CD40 and CD80 expression on CD14+ monocytes of the 3 independent experiments. The data represent mean ± SE.

View larger version (31K): [in a new window]   Figure 2. Comparison of monocytes, NK, and T-cell activation between aggressive periodontitis patients and non-periodontitis subjects (n = 17, each group). Whole-blood cultures were incubated with P. gingivalis LPS (0, 1, 3, 10 ng/mL) for 48 hrs. (A) Up-regulation of monocyte CD40, CD80, and CD86 expression was analyzed by flow cytometry. Each symbol represents MFI-fold increase of each individual. Horizontal lines are means. (B). Up-regulation of CD69 expression on NK and T-cells was analyzed by flow cytometry. Each symbol represents % positive cell-fold increase of each individual. Horizontal lines = means.

View larger version (12K): [in a new window]   Figure 3. Comparison of IL-1ß and PGE2 production between aggressive periodontitis and non-periodontitis subjects (n = 17, each group). Whole-blood cultures were incubated with 0, 1, 3, and 10 ng/mL of P. gingivalis LPS for 48 hrs and then assessed for IL-1ß and PGE2 production by ELISA. Results are the mean ± SE.