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Clonal Persistence of Oral Fusobacterium nucleatum in Infancy

¹ Anaerobe Reference Laboratory, National Public Health Institute (KTL), Helsinki, Finland;
² Faculty of Odontology, University of Iceland, Vatnsmyrarvegur 16 IS 101 Reykjavik, Iceland; and
³ Faculty of Dentistry, Kuwait University, Kuwait;

View larger version (10K): [in a new window]   Figure 1. The relationship between the total number of F. nucleatum isolates examined and the number of AP-PCR types detected in each sample. The equation of the line is y = 0.488 + log x (Rsq = 0.244; p = 0.001), where x is the number of isolates examined.

View larger version (78K): [in a new window]   Figure 2. Representatives of all AP-PCR types from Infant C. The upper part of the gel = primer C1, and the lower part of the gel = primer D8635. Lanes 1 and 18, 100-bp ladder; lanes 2–5, AP-PCR type 3; lane 6, AP-PCR type 5; lane 7, AP-PCR type 6; lane 8, AP-PCR type 2; lane 9, AP-PCR type 4; lane 10, AP-PCR type 7; lane 11, AP-PCR type 8; lanes 12–14, AP-PCR type 9; lanes 15–16, AP-PCR type 1; lane 17, negative control.

View larger version (11K): [in a new window]   Figure 3. Clonal diversity of oral F. nucleatum populations in 12 infants during their first 2 yrs of life. Each line represents distinct AP-PCR types (n = the number of isolates examined at each sampling). The dominating AP-PCR types (representing more than one-third of the examined isolates) are marked in bold. Broken lines indicate the periods during when the later-redetected types were missing.