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Dentonin, a Fragment of MEPE, Enhanced Dental Pulp Stem Cell Proliferation

¹ Box 0640, University of California, San Francisco, CA 94143-0640, USA;
² Peking University School of Stomatology, Beijing, China; and
³ Acologix, Emeryville, CA, USA;

View larger version (19K): [in a new window]   Figure 1. Western blot of DSP separated by SDS PAGE. Equal amounts of protein from pre-confluent DPSCs (PC), confluent (C) DPSCs, and cells with mineralizing nodules (M) were loaded into each lane. There was no detectable effect of Dentonin on the relative level of DSP secreted at each stage of culture.

View larger version (22K): [in a new window]   Figure 2. The effect of Dentonin on DPSC proliferation shows: (A) the rate of cell proliferation in Dentonin-treated cells increased relative to the control group (p 0.05); and (B) DPSC proliferation was most enhanced with Dentonin peptide, and was reduced when either the RGD or SGDG motifs were altered.

View larger version (12K): [in a new window]   Figure 3. Cell-cycle SuperArray analysis showed that, of the more highly expressed genes, P16 was down-regulated approximately two-fold in the presence of Dentonin. Genes expressed at lower levels included E6-AP and SUMO-, which were up-regulated more than 7 times by Dentonin.

View larger version (76K): [in a new window]   Figure 4. Osteogenesis SuperArray showed that multiple osteogenesis-related genes were expressed by DPSCs in culture. Most highly expressed (black arrows) are fibronectin, osteonectin, decorin, CBFA1, and integrin ß1. There was no measurable difference between mRNA from DPSCs exposed to Dentonin in culture, as opposed to control (unexposed) cells.