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Preparation and Characterization of Electrodeposited Calcium Phosphate/Chitosan Coating on Ti6Al4V Plates

¹ IsoTis S.A., Prof. Bronkhorstlaan 10-D, 3723 MB Bilthoven, The Netherlands;
² Biomaterials Research Group, Leiden University, The Netherlands; and
³ School of Stomatology, Wuhan University, People’s Republic of China;

View larger version (141K): [in a new window]   Figure 1. SEM micrographs of the ELD and ELDC coatings (0.1 g/L chitosan in solution). (a) In the ELD coating, note Ca-P globules and small plate-like crystals. (b,c,d) In the ELDC coating, note many chitosan aggregates around the Ca-P globules and interconnected to form a net (b). Some of them spread and integrated with calcium phosphate crystals (c,d). Ch, chitosan; CaP, calcium phosphate.

View larger version (42K): [in a new window]   Figure 2. Critical load forces of the ELD and ELDC coatings. The bars indicated the means ± SD (n = 3), and numbers in the brackets represent the chitosan concentration in solution. Note that the ELDC coatings exhibited lightly varied critical load forces at the first crack (Lc1) but higher critical load forces at the complete delamination (Lc2). No statistical differences were found on Lc1 or Lc2 (P > 0.05).

View larger version (210K): [in a new window]   Figure 3. SEM micrographs of goat bone marrow stromal cells attached to the ELD and ELDC coatings (0.2 g/L chitosan in solution) after 1 day’s (a,b) or 3 days’ (c,d,e,f) culture. Note cells differentiated to a polygonal shape with extending cytoplasmic processes adhering to the ELDC coating (c,d), in contrast to cells presenting flat and shaped more regularly attached to the ELD coating (a,b). Particularly, cells were found favorably attached to chitosan at higher magnification (e,f). C, cells; Ch, chitosan.