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Persistent Colonization with Tannerella forsythensis and Loss of Attachment in Adolescents

¹ Oral Biology and Pathology, School of Dentistry, University of Queensland, St Lucia, 4072, Australia; and
² Dental Health Unit, University of Manchester, Manchester, M15, 6SH, UK;

View larger version (94K): [in a new window]   Figure 1. Sensitivity and specificity of the PCR methodology. (A) Lanes 1-8 show a serial dilution of T. forsythensis ATCC 43037 starting at 107 bacteria. Lane 9 is an assay-negative control (H2O). The limit of sensitivity was estimated as 103 bacteria. (B) An agarose gel of PCR products of DNA extracted from two T. forsythensis strains and some of the panel of oral bacteria tested (not all negative results shown). Lane 1, DNA 100-base-pair marker; Lane 2, T. forsythensis ATCC 43037; Lane 3, T. forsythensis 7007; Lane 4, A. actinomycetemcomitans Y4; Lane 5, A. actinomycetemcomitans ATCC 29524; Lane 6, P. intermedia ATCC 25611; Lane 7, P. intermedia FDC 581; Lane 8, P. gingivalis FDC 381; and Lane 9, P. gingivalis ATCC 33277.

View larger version (22K): [in a new window]   Figure 2. Prevalence and carriage rate of T. forsythensis. (A) The prevalence of T. forsythensis was significantly (*p < 0.005) higher in the LOA+ subjects (filled bars) compared with LOA- subjects (unfilled bars) at every examination (based on an approximate normal test for differences between proportions of two independent groups). The carriage rate of T. forsythensis in subjects who had detectable levels of T. forsythensis at baseline is demonstrated in panel (B). Significantly (*p < 0.005) fewer (four of seven) LOA- subjects (unfilled bars) were demonstrated to maintain detectable levels of T. forsythensis longitudinally compared with 18 of 18 LOA+ subjects (filled bars).