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Localization of Porphyromonas gingivalis-carrying Fimbriae in situ in Human Periodontal Pockets

¹ Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1–8 Yamadaoka, Suita, Osaka 565-0871, Japan; and
² Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Aichi 464-8650, Japan;

View larger version (31K): [in a new window]   Figure 1. Localization of P. gingivalis and P. gingivalis-carrying fimbriae in human periodontal pockets. In panels A and B, each number indicates the sample number (Nos. 1–11). This is also shown in Table 1. (A) In all 6 anti-P. gingivalis-positive samples, the reactions are scattered throughout almost all of the 9 separate sites. (B) Positive reactions with the anti-P. gingivalis fimbriae serum are detected in 6 samples. In all 6 positive samples, the reactions are found in the cementum-attached plaque area at the bottoms of the periodontal pockets. In the epithelium-associated plaque area, the reactions for the fimbriae are observed only in 2 positive samples.

View larger version (109K): [in a new window]   Figure 2. TEM photographs of a positive control (A), an ultrastructural image (B), and immunogold staining images (C,D) of the PFZ. (A) Immunolabeled gold particles are observed along the fimbriae of a P. gingivalis cell, reacted with the anti-fimbriae serum. (B) Small numbers of bacterial cells, including a filamentous organism (arrow), attached to the DC covering acellular cementum (C), and aggregated with other cells. (C,D) Gold particles (arrows) can be observed along the fimbriae of the cells, in close contact with the DC in the PFZ. DC in panels B, C, and D: dental cuticle. Bar = 0.1 µm (A); bar = 0.5 µm (B-D).