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Epitope Mapping of Porphyromonas gingivalis Heat-shock Protein and Human Heat-shock Protein in Human Atherosclerosis

¹ Department of Periodontology and Research Institute for Oral Biotechology, School of Dentistry, ² Department of Thoracic and Cardiovascular Surgery, School of Medicine, ³ Department of Molecular Biology, College of Natural Sciences, ⁴ Department of Pharmacology, and ⁵ Department of Microbiology, School of Medicine, Pusan National University, 1–10, Ami-Dong, Seo-Ku, Pusan 602–739, Korea;

View larger version (36K): [in a new window]   Figure 1. (A) Western immunoblot pattern demonstrating the cross-recognition of P. gingivalis HSP60 and human HSP60 by sera obtained from six atherosclerosis patients (A1-A6). This was not observed in healthy control subjects (N1-N6). Pairs of two lanes represent serum reactivity of one subject, left panels being against P. gingivalis HSP 60 (P), right panels being against human HSP 60 (H), respectively. (B) In addition, mouse anti-P. gingivalis HSP60 antisera recognized P. gingivalis HSP and cross-reacted with all the HSPs induced by heat-shock treatment of putative periodontopathogenic bacteria tested. Pg, P. gingivalis; Aa, A. actinomycetemcomitans; Bf, B. forsythus; Fn, F. nucleatum; Pi, P. intermedia; Td, T. denticola.

View larger version (38K): [in a new window]   Figure 2. Bar diagram representing optical densities as a measure of positive reactivity of patient sera to 108 synthetic peptides spanning whole molecules of P. gingivalis HSP60 and human HSP60, respectively. The number indicates each patient. Fifteen antigenic peptides that showed positive signals in more than four out of six patients were designated as B-cell epitopes of P. gingivalis HSP60. In the same manner, B-cell epitopes of human HSP60 were identified.