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Progress of Cell Proliferation in Striated Muscle Tissues during Development of the Mouse Tongue

Department of Pharmacology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan;

View larger version (13K): [in a new window]   Figure 1. Electrophoretic gel pattern (A) of cyclin D1 cDNA standard and its competitor after competitive PCR, and the standard curve (B). Chronological changes in the expression level of cyclin D1 mRNA in mouse tongues at embryonic days (E) 11, 13, 15, 17, and birth as analyzed by competitive RT-PCR (C). Each point with its vertical bar represents the mean ± 1 SD of 5 samples. The vertical axis is expressed as a percentage of the mean value at E11.

View larger version (154K): [in a new window]   Figure 2. Immunostaining images for PCNA (A-C) and desmin (D) in the somites 2, 3 (A), 4, 5 (B), and 3 (C,D) of the E9 mouse embryo. The diagrammatic representations show sagittal sections of tongues at E11 (E) and E13 ~ birth (F), viewed from the buccal side. The dotted rectangles indicate the regions in which proliferating cell nuclear antigen (PCNA)-labeling indices were obtained. Immunostaining images for PCNA (G,H,K-N) and fast myosin heavy-chain (I,O-R) in the whole portion of the E11 tongue (G,I), and in the anterior (K,L,O,P), middle (M,Q), and posterior (N,R) regions of E13 tongues. A control staining image of a whole portion of an E11 tongue treated with normal mouse IgG instead of the antibodies (J). H, L, and P are higher-magnification images of the regions indicated by the rectangles in G, K, and O, respectively. White lines in L contour the areas which lack the PCNA-positive cell nuclei.

View larger version (123K): [in a new window]   Figure 3. Immunostaining images for PCNA (A-H) and fMHC (I-L) in the anterior (A,E,I), middle (B,F,J), and posterior (C,D,G,H,K,L) regions of E15 (A-D) and E17 (E-L) tongues. Chronological changes in the PCNA-labeling index in mouse tongues between E11 and birth (M). D, H, and L are higher-magnification images of the regions indicated by the rectangles in C, G, and K, respectively.

View larger version (12K): [in a new window]   Figure 4. The stages in which commitment, migration, proliferation, differentiation, and maturation occur most actively during the development of the mouse tongue. Data for the commitment, migration, differentiation, and maturation stages are from the results of Mayo et al.(1992), Yamane et al.(2000), and Amano et al.(2002).