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Collagen Cross-linking and Ultimate Tensile Strength in Dentin

¹ Department of Operative Dentistry, CB# 7455, and ² Dental Research Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7455, USA;

View larger version (17K): [in a new window]   Figure 1. Sample preparation for microtensile test. The dentin samples for biochemical analyses were collected from the same area where the microtensile test was performed. The tooth was cut parallel to its long axis (a), indicated by a thin dotted line, and the slices were further divided into crown and root, indicated by a thick dotted line. The microtensile test was performed either parallel (b) or perpendicular (c) to the direction of the dentinal tubules.

View larger version (15K): [in a new window]   Figure 2. Collagen cross-link analysis in crown and root dentin (n = 9). (a) Cross-link precursor aldehydes, (b) reducible cross-links, and (c) pyridinoline cross-links. Asterisks indicate significant differences (p < 0.005, p < 0.001, and p < 0.005, respectively) between the two groups.

View larger version (147K): [in a new window]   Figure 3. Direction of the collagen fibrils (arrows) in relation to the dentinal tubules in an undemineralized crown specimen (SEM at 20,000X).