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Effects of TGF-ß on Hyaluronan Anabolism in Fibroblasts Derived from the Synovial Membrane of the Rabbit Temporomandibular Joint

Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan;

View larger version (21K): [in a new window]   Figure 1. Amounts of hyaluronan (HA) in synovial membrane fibroblast cultures treated with and without transforming growth factor beta 1 (TGF-ß1) measured by means of high-performance liquid chromatography and differential refractometer. (A) < 300 kDa HA in the conditioned medium (mean ± SD, n = 3). (B) < 300 kDa HA in the cell layer (mean ± SD, n = 3). (C) 300 ~ 1900 kDa HA in the conditioned medium (mean ± SD, n = 3). (D) 300 ~ 1900 kDa HA in the cell layer (mean ± SD, n = 3). (E) > 1900 kDa HA in the conditioned medium (mean ± SD, n = 3). (F) > 1900 kDa HA in the cell layer (mean ± SD, n = 3). The amount of HA with TGF-ß1 treatment for 12 to 72 hrs was compared with the non-treatment control by Student’s t test. *p < 0.05, **p < 0.01.

View larger version (17K): [in a new window]   Figure 2. Hyaluronan synthases (HASs) mRNA levels in synovial membrane fibroblast cultures treated with and without transforming growth factor beta 1 (TGF-ß1) determined by a real-time polymerase chain-reaction analysis (PCR). (A) Dose-dependent effects of TGF-ß1 on HAS2 mRNA levels (mean ± SD, n = 3). (B) Time-dependent effects of TGF-ß1 on HAS2 mRNA levels (mean ± SD, n = 3). (C) Dose-dependent effects of TGF-ß1 on HAS3 mRNA levels (mean ± SD, n = 3). (D) Time-dependent effects of TGF-ß1 on HAS3 mRNA level (mean ± SD, n = 3). Data were normalized relative to the expression of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) and shown as a fold increase in mRNA expression. The dotted line indicates the control level of HAS mRNAs. Fold increases in the expression of HASs mRNAs by TGF-ß1 treatment were compared with the control levels dose- (0.1 ~ 100 ng/mL) and time- (1 ~ 12 hrs) dependently by Student’s t test. *p < 0.05, **p < 0.01.