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Salivary Esterase Activity and Its Association with the Biodegradation of Dental Composites

¹ Restorative Discipline and
² Biomaterials Discipline, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada M5G 1G6;

View larger version (15K): [in a new window]   Figure 1. Activity profiles for CE (A) and PCE (B) with para-nitrophenylacetate (p-NPA), ortho-nitrophenylacetate (o-NPA), para-nitrophenylbutyrate (p-NPB), ortho-nitrophenylbutyrate (o-NPB), and butyrylthiocholine iodide (BTC). All data are reported as mean ± standard error. N = 3. Standard deviations for CE range from 0.01 to 0.16 units/µg protein, and for PCE from 0.0002 to 0.003 units/µg protein.

View larger version (22K): [in a new window]   Figure 2. Esterase-like activity measured in human saliva, collected from different subjects and measured with para-nitrophenylacetate (p-NPA), ortho-nitrophenylacetate (o-NPA), para-nitrophenylbutyrate (p-NPB), and ortho-nitrophenylbutyrate (o-NPB), (pH 7.0 at 25°C).

View larger version (12K): [in a new window]   Figure 3. The inhibition effect of PMSF (at PMSF concentrations to achieve 40% reduction in activity with respect to the substrates below) on the activities of CE and PCE (pH 7.0 at 25°C). The activities were measured with para-nitrophenylacetate and butyrylthiocholine as substrates for CE and PCE, respectively. PMSF concentration for CE was 1 mM and for PCE was 0.5 mM. All data are reported as mean ± standard error. N = 3. Standard deviations for CE-incubated groups range from 5.2 to 7.9%, and for PCE groups from 3.8 to 5.3%.

View larger version (16K): [in a new window]   Figure 4. Inhibition of CE and PCE catalyzed biodegradation for cured composite resin samples by PMSF, following 16 days’ incubation (pH 7.0 at 37°C). (A) Inhibition of methacrylic acid production. (B) Inhibition of bisHPPP production. PMSF concentration for CE was 0.1 mM and for PCE was 0.05 mM. All data are reported as mean ± standard error. N = 3. Standard deviations for MA-analyzed product range from 5.9 to 8.3%, and for bisHPPP products from 4.0 to 11.8%.