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Spectroscopic Imaging of Mineral Maturation in Bovine Dentin

¹ Hospital for Special Surgery, New York, NY, USA;
² Dental Research Center, University of North Carolina at Chapel Hill, NC, USA;

View larger version (54K): [in a new window]   Figure 1. Areas analyzed by FTIRI in bovine incisors: 3rd-trimester I2 developing incisor (a) and year-old I4 incisor (b). Young dentin (cervical) and older dentin (mid-crown and incisal) areas are noted. Rectangles indicate areas analyzed. Cervical and incisal areas analyzed as controls also indicated on year-old incisor (c). Spectra extracted from mantle dentin area of fields analyzed in incisor (a) at various distances from the cervix of the tooth [as indicated in (a)] and in incisor (b, stippled spectrum). 1,3 PO43+ and Amide I bands noted. Pulp, dentin, and enamel are shown in (a). Bar = 0.5 mm.

View larger version (54K): [in a new window]   Figure 2. FTIR imaging analysis of 400 x 400 µm consecutive fields from cervical, mid-crown, and incisal parts of the fetal calf incisor shown in Fig. 1a. Composites of (a) actual field optical micrographs, (b) mineral:matrix ratio FTIR images, and (c) crystallinity FTIR images. Distribution of mineral:matrix and crystallinity values along a predentin-to-enamel line for the field marked with an asterisk is shown in Fig. 4a. P = Pulp, D = Dentin, E = Enamel. Bar represents 0.5 mm.

View larger version (59K): [in a new window]   Figure 3. FTIR imaging analysis of 400 x 400 µm consecutive fields from cervical and incisal parts for the year-old incisor shown in Fig. 1b. (a) Optical micrographs, (b) mineral:matrix ratio FTIR images, and (c) crystallinity FTIR images. Distribution of mineral:matrix and crystallinity values along a predentin-to-dentin line for the field marked with an asterisk is shown in Fig. 4b. P = Pulp, D = Dentin. Bar represents 1 mm.

View larger version (41K): [in a new window]   Figure 4. Changes in spectral parameters across selected regions of the fetal (a) and year-old incisor (b), shown by white lines in the Fig., show significant linear mineral:matrix increases in the mineralization front and the DEJ, with only a slight increase in the crystallinity parameter in the same areas. The linear regions analyzed are shown in the micrographs of the fields marked with an asterisk in Figs. 2a and 3a. P = Pulp, D = Dentin, E = Enamel. Crystallinity values for enamel pixels were not computed.