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Expression of MMP-8 and MMP-13 Genes in the Periodontal Ligament during Tooth Movement in Rats

Division of Orthodontics and Dentofacial Orthopedics,
¹ Division of Molecular Biology, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku Sendai, 980-8575, Japan;

View larger version (147K): [in a new window]   Figure 1. Photomicrographs of horizontal sections of maxillary first molars from control and experimental animals (hematoxylin and eosin stain). A, D, G, J, and M: Middle-buccal root of first molars at days 0, 2, 4, 7, and 14 (original magnification, x40; Bar in M = 150 µm). B, E, H, K, and N: Tension side at days 0, 2, 4, 7, and 14 (original magnification, x200; Bar in O = 150 µm). C, F, I, L, and O: Compression side of moving teeth at days 0, 2, 4, 7, and 14 (original magnification, x200). Hyalinized tissues were observed at days 2 and 4, shown in D and F. AB, alveolar bone; CEM, cementum; PDL, periodontal ligament; HY, hyalinized tissue; OC, osteoclast; and BV, blood vessel. n = 3.

View larger version (101K): [in a new window]   Figure 2. Photomicrographs of ISH images of maxillary first molar roots stained for type I collagen (Col1a1) and type III collagen (Col3a1) are indicated (n = 3). Left lane (A, C, E, and G): type I collagen. Right lane (B, D, F and H): type III collagen. A and B, control; C and D, day 4; E and F, day 7; and G and H, day 14. Original magnification: x100. Bar in H = 150 µm.

View larger version (135K): [in a new window]   Figure 3. ISH images of MMP-8 (Mmp-8) and MMP-13 (Mmp-13) from control samples (n = 3) and from tension (n = 3) and compression sides (n = 3). A through E and K through O are ISH images of MMP-8, and F through J and P through T are of MMP-13. The two left columns are from controls and from the tension side; the two right columns are from the compression side. Arrowheads indicate the positive signals. Original magnification: x200. Bar in T = 150 µm. K and P indicate the hyalinized tissue in the highly compressed area, and L and Q indicate non-hyalinized tissue. AB, alveolar bone; CEM, cementum; PDL, periodontal ligament; HY, hyalinized tissue; OC, osteoclast; and BV, blood vessel.

View larger version (35K): [in a new window]   Figure 4. Densitometric results from semi-quantitative RT-PCR analysis (mean ± standard deviation). The magnitude-dependent effects of tension (A) and compression (B) on mRNA expression of MMP-8 and MMP-13 are indicated. Open bars, MMP-8; solid bars, MMP-13 (A and B: n = 6). Time course changes in the relative expressions of MMP-8 (C) and MMP-13 (D) are indicated. Open bars, control non-compressed cultures; solid bars, compressed cultures at 0. 2kPa (C and D, n = 4). The effects of conditioned media from cultures compressed or not compressed for 1 and 3 days on the expression of MMP-8 (E) and MMP-13 (F) were measured at 1 and 3 days of culture. Open bars, control, noncompressed media; solid bars, compressed media (E and F, n = 3). CM, conditioned media. **P < 0.01, *P < 0.05.