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Figure 1. Antitumor effects induced by immunization with KLN-205 vaccine. The mice were immunized with KLN-205 vaccine, B16 vaccine, Sq1979 vaccine, KLN-205 treated with GA, OK-432 treated with GA, or OK-432 alone by means of a 100-µL syringe and a 30-gauge needle, once a wk for 3 wks. Control-group mice were injected with saline in the same manner. *Significant difference between the groups of KLN-205 vaccine and saline (control) (p < 0.05). One wk after final injection, all mice were inoculated with KLN-205 cells. (A) Suppression of tumor incidence by the KLN-205 vaccine. (B) Suppression of tumor growth by the KLN-205 vaccine. We monitored tumor size by measuring the longest diameter (mm) and the perpendicular diameter (mm) of the mass, and scored it by using the formula 0.5 x a x b2 (mm3), a being the longer diameter and b being the shorter diameter. (C) Suppression of mortality rate by the KLN-205 vaccine. Kaplan-Meier curves showing the probability of survival were constructed for immunized mice groups and control mice groups. (D) Suppression of KLN-205 tumor growth by post-immunization with KLN-205 vaccine. Arrows represent each post-immunization. One day after inoculation with KLN-205, mice were post-immunized 3 times with KLN-205 vaccine, B16 vaccine, Sq1979 vaccine, KLN-205 treated with GA, OK-432 treated with GA, or OK-432 alone. Tumor size was then monitored as described above.
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