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Intrinsic Regulation of CGRP Release by Dental Pulp Sympathetic Fibers

¹ Department of Endodontics, UTHSCSA School of Dentistry, Mail Code 7892, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900;
² Division of Endodontics, University of Minnesota School of Dentistry; and
³ Department of Oral Medicine, University of Washington School of Dentistry;

View larger version (14K): [in a new window]   Figure 1. Effect of norepinephrine administration on capsaicin-evoked release of immunoreactive calcitonin gene-related peptide (iCGRP) from in vitro-superfused dental pulp. Bovine dental pulp was collected, sliced into 200-µm2 slices, and superfused with oxygenated Krebs’ buffer. Levels of iCGRP released from dental pulp were collected over a seven-minute fraction and measured by radioimmunoassay. Norepinephrine (3 nM) or vehicle was administered from fractions 0–28 min, and capsaicin (10 µM) was administered to all chambers over a 21- to 28-minute period. Data are calculated as % increase over baseline 100 x (peak release - baseline)/baseline. Error bars are SEM. **p < 0.01 vs. vehicle. N = 6/group.

View larger version (11K): [in a new window]   Figure 2. Effect of pre-treatment with -adrenoceptor antagonists (phentolamine and phenoxybenzamine, 10 µM) or vehicle on spontaneous release of iCGRP from superfused dental pulp. Levels of iCGRP were measured as described in the legend to Fig. 1. Error bars are SEM. **p < 0.01 vs. vehicle. N = 8–16/group.

View larger version (16K): [in a new window]   Figure 3. Effect of guanethidine treatment on capsaicin-evoked release of iCGRP from superfused dental pulp. Pulpal tissue was treated with vehicle or phentolamine 10 µM for 7 min, and then either vehicle or guanethidine, 100 µM, was administered for an additional 21 min before stimulation with capsaicin (10 µM). Levels of iCGRP were measured as described in the legend to Fig. 1. Error bars are SEM. **p < 0.01 vs. both other groups. N = 4–5/group.

View larger version (15K): [in a new window]   Figure 4. Effect of reserpine treatment on capsaicin-evoked release of iCGRP from superfused dental pulp. Pulpal tissue was treated with vehicle or phenoxybenzamine, 10 µM, for 7 min, and then either vehicle or reserpine, 100 µM, was administered for an additional 21 min before stimulation with capsaicin (10 µM). Levels of iCGRP were measured as described in the legend to Fig. 1. Error bars are SEM. **p < 0.01 vs. both other groups. N = 5–6/group.