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Regulation of Osteoprotegerin Gene Expression in Dental Follicle Cells

Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803;

View larger version (46K): [in this window] [in a new window]   Figure 1. (A) Ethidium-bromide-stained gels of RT-PCR products for OPG, PTHrP-R, and CSF-1 after incubation of DFC with different concentrations of PMA. Note that PMA up-regulates OPG gene expression but down-regulates PTHrP-R and CSF-1 expression. In these and all subsequent graphs, the bars labeled with the same letters are not significantly different according to a LSD t test (P 0.05). (B) RT-PCR products for OPG, PTHrP-R, and CSF-1 after incubation of DFC with 50 ng/mL PMA over different time periods. Again, PMA enhances OPG expression (six-hour maximal effect) and decreases PTHrP-R and CSF-1 (six-hour maximum effect).

View larger version (50K): [in this window] [in a new window]   Figure 2. (A) RT-PCR products for OPG, PTHrP, and CSF-1 after incubation of DFC with different concentrations of dbcAMP. Note that dbcAMP down-regulates OPG gene expression but conversely up-regulates PTHrP-R and CSF-1. (B) RT-PCR products for OPG, PTHrP-R, and CSF-1 after incubation of DFC with 5 µg/mL of dbcAMP over different time periods. Again, dbcAMP reduces OPG expression (six-hour maximum effect) and increases PTHrP-R (24-hour maximum) and CSF-1 (12-hour maximum) expression.

View larger version (29K): [in this window] [in a new window]   Figure 3. (A) RT-PCR products for PKC- after incubation of DFC with PMA in either a concentration-dependent or time-dependent study. Note that PMA up-regulates PKC- expression. (B) RT-PCR products for PKC- after incubation of DFC with PTHrP in either a concentration-dependent or time-dependent study. PTHrP down-regulates PKC- expression. (C) RT-PCR products for PKC- after incubation of DFC with CSF-1 in either a concentration-dependent or time-dependent study. CSF-1 down-regulates PKC- expression.

View larger version (36K): [in this window] [in a new window]   Figure 4. Immunostaining of DFC for OPG in the absence of PMA (panel A) and after incubation with PMA at a concentration of 100 ng/mL for 12 hrs (panel B). Note the enhanced brown immunostain in the cells incubated with PMA in panel B.

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