|
Sign In to gain access to subscriptions and/or personal tools.
|
Journal of Dental Research, Vol. 82, No. 2,
91-95 (2003)
DOI: 10.1177/154405910308200203
Transient Co-localization of c-Jun N-terminal Kinase and c-Jun with Heat Shock Protein 70 in Pulp Cells during Apoptosis
C. Kitamura1,*,
Y. Ogawa1,
T. Nishihara2,
T. Morotomi1 and
M. Terashita1
1 Departments of Operative Dentistry and Endodontics, and
2 Oral Microbiology, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita, Kitakyushu 803-8580, Japan;
View larger version (119K):
[in this window]
[in a new window]
|
Figure 1. Distribution patterns of p-JNK, p-c-Jun, and HSPs in non-treated and one-hour groups. (a-h) Non-treated group. Signals of neither p-JNK nor p-c-Jun were detected in odontoblasts (a,c). Signals of p-JNK were not observed in any pulp cells (a,b), whereas signals of p-c-Jun (white arrowheads) were in only a few pulp cells close to odontoblasts (c). HSP70 (black arrows) and HSP27 (white arrows) were detected in the cytoplasm of most odontoblasts (ob) and some pulp cells (e-h). (i-p) One hour after injury. Signals of p-JNK (black arrowheads), as well as p-c-Jun (white arrowheads), were observed in a few pulp cells, but not in odontoblasts (i-l). There were no changes in the intracellular localization of HSP70 (black arrows) and HSP27 (white arrows) both in odontoblasts and in pulp cells (m-p). OBR, odontoblast region; PC, pulp cells; ob, odontoblasts. Scale bar = 10 µm.
|
|
View larger version (154K):
[in this window]
[in a new window]
|
Figure 2. Distribution patterns of p-JNK, p-c-Jun, and HSPs one day after injury. (a-c) Signals of p-JNK (black arrowheads) were frequently observed in nuclei of pulp cells, but not in odontoblasts. (d-f) Signals of p-c-Jun (white arrowheads) were also observed in many pulp cells and some odontoblasts. (g-i). HSP70 translocated into the nucleus from the cytoplasm (black arrows) only in pulp cells, not in odontoblasts. (j-l) The distribution pattern of HSP27 did not change (white arrows). OBR, odontoblast region; PC, pulp cells; ob, odontoblasts. Scale bars = 10 µm (low magnification) and 5 µm (high magnification).
|
|
View larger version (118K):
[in this window]
[in a new window]
|
Figure 3. Distribution patterns of p-JNK, p-c-Jun, and HSPs 4 and 14 days after injury. (a-h) Four days after injury. Signals of p-JNK (black arrowheads in a) and p-c-Jun (white arrowheads in c) were detected only in nuclei of some pulp cells close to odontoblasts, not in pulp cells far from odontoblasts (b,d). HSP70 (black arrows in f) relocalized from the nucleus to the cytoplasm of pulp cells. HSP27 (white arrows in h) was strongly detected in the cytoplasm of pulp cells. There were no changes in distribution patterns of HSPs in odontoblasts (e,g). (j-q) Fourteen days after injury. Both signals of p-JNK (black arrowheads in j) and p-c-Jun (white arrowheads in l) were observed in pulp cells close to odontoblasts, not in other pulp cells (k,m). Levels of HSP70 (black arrows in n,o) and HSP27 (white arrows in p,q) in odontoblasts and pulp cells returned to those in non-treated molars. OBR, odontoblast region; PC, pulp cells; ob, odontoblasts. Scale bar = 10 µm.
|
|
View larger version (101K):
[in this window]
[in a new window]
|
Figure 4. (a-c) TUNEL staining with immunostaining one day after injury. In some TUNEL-positive cells (blue signals), signals of p-JNK and p-c-Jun were detected (black arrowheads in a,b). Signals of HSP70 were detected only in TUNEL-negative cells (black arrows in c). (d,e) Double-immunostaining of p-JNK, p-c-Jun, and HSP70 in one day. In some pulp cells, the co-localization of HSP70 with p-JNK (d) and p-c-Jun (e) was observed (yellow signals indicated by white arrows). Pulp cells predominantly expressing HSP70 were also observed (red signals indicated by white arrowheads). Scale bar = 5 µm.
|
|
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
|
|
