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Relative Levels of mRNA Encoding Enamel Proteins in Enamel Organ Epithelia and Odontoblasts

¹ Department of Periodontics and Endodontics and
² Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan;

View larger version (44K): [in this window] [in a new window]   Figure 1. Schematic drawing of ameloblast and odontoblast tissue preparations. SA, secretory ameloblast (enamel organ epithelia; EOE) layer; MA, maturation ameloblast (EOE) layer; YO, young odontoblast layer; MO, mature odontoblast layer.

View larger version (59K): [in this window] [in a new window]   Figure 2. Detection of mRNAs encoding GAPDH, amelogenin, enamelin, sheathlin, enamelysin, and KLK4 after 25 cycles of RT-PCR. Amelogenin, enamelin, sheathlin, enamelysin, and KLK4 PCR products were amplified from the cDNA template and separated by electrophoresis on a 4.5% polyacrylamide gel. M, molecular-size standard, PhiX174 DNA-Hae III (New England Biolabs); SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer; PO, pre-odontoblast layer; DP, dental pulp cells; EO, erupted first molar odontblast layer.

View larger version (32K): [in this window] [in a new window]   Figure 3. Quantitative PCR of amelogenin, enamelin, sheathlin, enamelysin, KLK4, and GAPDH mRNAs isolated from the various cell layer samples. SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer. The most typical pattern was shown. The vertical axis shows relative amounts, and the horizontal axis shows cycles.

View larger version (31K): [in this window] [in a new window]   Figure 4. The relative amounts of amelogenin (A), enamelin (B), sheathlin (C), enamelysin (D), and KLK4 (E) mRNA in the cell layer samples, after normalization with the amounts of the GAPDH mRNA. SA, secretory ameloblast layer; MA, maturation ameloblast layer; YO, young odontoblast layer; MO, mature odontoblast layer. The vertical axis shows relative amounts. The results were expressed as means ± SD of three samples.

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