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Journal of Dental Research, Vol. 81, No. 7, 472-476 (2002)
DOI: 10.1177/154405910208100708

Two Modes of Nanoleakage Expression in Single-step Adhesives

F.R. Tay1, D.H. Pashley2,* and M. Yoshiyama3

1 Pediatric Dentistry and Orthodontics, The University of Hong Kong, The Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, SAR, China;
2 Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA 30912-1129, USA; and
3 Department of Operative Dentistry, Okayama University Graduate School of Medicine and Dentistry, 2-5-1, Shikato-cho, Okayama 700-8525, Japan;


Figure 1
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  		Figure 1. Transmission electron microscopy of the reticular mode of nanoleakage expression along the resin-dentin interfaces of single-step, self-etch adhesive after immersion in conventional silver nitrate. (A) Prompt L-Pop. (B) Etch&Prime 3.0. (C) One-Up Bond F. (D) Reactmer Bond. Unstained hybrid layers (H) that were difficult to recognize were indicated between two open arrows. Despite variation in their acidity, a similar reticular mode of nanoleakage expression was consistently observed in the 4 self-etch adhesives. Within the hybrid layers, a horizontal network of electron-dense silver deposits (pointers) could be found around the unstained collagen fibrils. An additional series of vertically oriented silver deposits (open arrowheads) extended from the surface of the hybrid layers into the overlying adhesive layers (A). D, undemineralized intertubular dentin; T, dentinal tubule. (For Reactmer Bond: G, fluoroaluminosilicate glass fillers; black arrowhead, fumed silica fillers).

 

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  		Figure 2. Transmission electron microscopy of an additional spotted mode of nanoleakage expression along the resin-dentin interfaces of single-step, self-etch adhesive after immersion in basic, ammoniacal silver nitrate. (A) Prompt L-Pop. (B) Etch&Prime 3.0. (C) One-Up Bond F. (D) Reactmer Bond. Unstained hybrid layers (H) that were difficult to recognize were indicated between two open arrows. The spotted mode of nanoleakage expression consisted of isolated spots of silver grains (pointers) that were dispersed within the hybrid layers and adhesive layers (A). The reticular mode of nanoleakage expression that was previously observed in conventional silver nitrate could also be seen. For One-Up Bond F (Fig. 2CGo), vertical streaks of silver deposits (open arrowhead) that were vaguely identified from Fig. 1CGo as a result of the sectioning plane of the specimen were more pronounced. D, intertubular dentin; P, peritubular dentin; T, dentinal tubule; G, glass fillers in Reactmer Bond.

 

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