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Hormonal Regulation of Androgen Receptor Messenger Ribonucleic Acid Expression in Human Tooth Pulp

¹ St. Louis University Center for Advanced Dental Education, Department of Endodontics, St. Louis, MO; and
² Southern Illinois University School of Dental Medicine, Department of Applied Dental Medicine, 2800 College Ave., Alton, IL 62002;

View larger version (19K): [in this window] [in a new window]   Figure 1. Gender- and age-correlated differences in AR mRNA expression in human tooth pulp. RNA was extracted from pulp tissue that was harvested from non-carious third molars (age, 18-55 yrs), and semi-quantitative RT-PCR was conducted to determine the relative abundance of AR mRNA. Values shown represent the ratio of AR:GAPDH in relative units. Bars represent the mean ± SEM of three independent experiments, and significant differences (P 0.05) in AR mRNA content among the subject groups are indicated by different letters.

View larger version (40K): [in this window] [in a new window]   Figure 2. ERβ expression in tooth pulp. Cultures of pulp cells were prepared and incubated for 48 hrs. RT-PCR was used to screen for ERβ mRNA. The arrow indicates the predicted 439-bp ERβ amplicon. Lane 1, PhiX 174 RF HaeIII DNA standard; lane 2, negative control; lanes 3-4, RNA from independent pulp cell cultures.

View larger version (26K): [in this window] [in a new window]   Figure 3. Steroid hormone-dependent regulation AR mRNA content in tooth pulp. Semi-quantitative RT-PCR was used to determine relative differences in AR mRNA content following treatment with E2 (panel a), androstenedione (panel b), testosterone (panel c), and DHT (panel d). Values shown represent the ratio of AR:GAPDH in relative units. Bars represent the mean ± SEM of three independent experiments. Significant differences (P 3; 0.05) in AR mRNA levels as a result of time in vitro and treatment are indicated by different letters.

View larger version (26K): [in this window] [in a new window]   Figure 4. The effect of HGF on AR mRNA in tooth pulp. (Panel a) At 48 hrs in vitro, RT-PCR was used to screen pulp cells for c-Met mRNA. A representative gel shows the presence of c-Met mRNA. The arrow indicates the position of the predicted 222-bp c-Met amplicon. Lane 1, 100-bp DNA ladder standard; lanes 1-3, RNA from three independent pulp cell cultures. (Panel b) Pulp cells were incubated in the presence and absence of HGF for 24 and 48 hrs. Semi-quantitative RT-PCR was used to measure relative differences in AR mRNA content. Values shown represent the ratio of AR:GAPDH in relative units. Bars represent the mean ± SEM of three independent experiments. Significant differences (P 3; 0.05) in AR mRNA levels as a result of time and treatment are indicated by different letters.

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