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Expression of N-acetyltransferases in Periodontal Granulation Tissue

¹ Department of Pharmacology,
² Department of Anatomy, and
³ Department of Human Genetics, Ernst Moritz Arndt University Greifswald, F.-Loeffler-Str. 23d, D-17487 Greifswald; and
⁴ Dental Clinics, Department of Periodontology, Rotgerberstr. 8, D-17487 Greifswald, Germany;

View larger version (35K): [in a new window]   Figure 1. NAT expression in granulation tissue. (A) RT-PCR products representing mRNA: N1-NAT1, N2-NAT2, G-GAPDH, each individual in three lanes from left to right. M, length standards; L, liver; arrows indicate approx. molecular weight in kDa. No. 64 – male, age 37, non-smoker, moderate periodontitis. No. 69 – recall. No. 65 – male, age 34, non-smoker, advanced periodontitis. No. 70 – recall. No. 76 – female, age 36, non-smoker, advanced periodontitis. No. 77 – male, age 45, 20 cigarettes/day, moderate periodontitis. (B) Western blots of NAT2 in gingival granulation tissues (lanes): No. 1 – female, age 36, non-smoker, advanced periodontitis; No. 2 – female, age 56, non-smoker, moderate periodontitis; No. 95 – female, age 17, non-smoker, local early-onset periodontitis (EOP); No. 97 – male, age 34, 20 cigarettes/day, advanced periodontitis.

View larger version (89K): [in a new window]   Figure 2. Immunohistochemical detection of N-acetyltransferases NAT1 (left column, A-C-E) and NAT2 (right column, B-D-F) in inflamed (top, A + B) or normal (middle, B + C) gingival tissue, control with pre-immune serum (bottom, E + F). Arrows indicate endothelial cells; bars represent 50 µm.